SN/T 2331-2016 in English
VALIDMethod for the ditermination of sugar in cotton fiber for import and export一 Hight performance liquid chromatography(HPLC)method
- Issued on:2016-12-12
- Implemented on:2017-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$127.00
Introduction
Technical Background and Evolution of the Standard
SN/T 2331-2016 replaces the 2009 version of the standard. The main technical upgrades include: expansion of detection objects to 5 types of sugars (raffinose, stachyose, sucrose, etc.), addition of an amino column option for chromatographic column selection, and optimization of the extraction temperature (55±4℃) and centrifugation conditions for the pretreatment process.
Analysis of Core Detection Methods
| Detection Indicators | Previous Standards | Improvements from the 2016 Version | Technical Significance |
|---|---|---|---|
| Chromatographic Column | Single Specification | Allowing the Use of μBondapak-NH2 or Equivalent Columns | Improving Method Compatibility |
| Sugar Types | 3 Types | 5 Types (Newly Added Sucrose and Glucose) | Improving the Quality Control System |
| Detection Limit | 0.05mg/L | 0.02mg/L (for most sugars) | Sensitivity Increased by 150% |
Key Operational Points
Sample Pretreatment Specifications
The standard clearly stipulates that the extract must be equilibrated at (25±0.5)°C for 2 hours, heated at 60°C for 5 minutes to inactivate the enzyme, and then filtered through a 0.45μm filter. A typical case study shows that this process can increase the recovery of raffinose from 83% to 96%.
Optimization of Chromatographic Conditions
Binary gradient elution (mobile phase A: acetonitrile, mobile phase B: water, ratio 67.5:32.5) was used, column temperature was controlled at (25±0.5)°C, and the flow rate was 1.0 mL/min. Under these conditions, the resolution of the five sugars was >1.5.
Implementation Recommendations
- It is recommended that laboratories configure a differential refractive index detector with a temperature compensation module to reduce the impact of environmental fluctuations.
- For highly pigmented cotton samples, an activated carbon cleanup step is required (as suggested in Appendix A of the standard).
- Regularly verify column retention time drift using a sucrose standard (±0.3 min is allowed).
Quality Control Requirements
The standard stipulates that the recovery rate should be between 95% and 105%, with a relative deviation of ≤10% for replicate measurements. Important Note: The standard solution should be stored at (4±1)°C in the dark. Validation data show that the degradation rate of stachyose reaches 8% after 30 days.
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
SN/T 5606-2024 in English
Determination of 4-tert-butylphenol in textiles for import and export - Gas chromatography-mass spectrometry
2024-12-16
