SN/T 4675.16-2016 in English

Preparation of standard solutions

The standard requires the accurate preparation of gradient standard solutions (0.10-5.0μg/mL), including:

• Stock solution (1.0 mg/mL): weigh 50.7 mg of fumaric acid standard
• Intermediate solution (10μg/mL): take 0.5 mL of stock solution and dilute to 50 mL
• The working solution must be prepared and used immediately and stored at 4℃ for no more than 3 days

Method validation data

Standard validation shows:

• Spike recovery: 84.8%-106.5%
• Relative standard deviation (RSD): 2.02%-5.19%
• Linear range: 0.10-5.0μg/mL (r²≥0.999)

Laboratory Implementation Recommendations

1. Mass spectrometer tuning requires optimization of collision energy (CE) to ensure that the characteristic ion abundance ratio meets the requirements of Appendix B
2. A matrix-matched standard curve should be provided with each batch of samples.
3. It is recommended to use the isotope internal standard method to improve quantitative accuracy.

Standard Update Tips

This standard references GB/T 1.1-2009 Guidelines for Standardization. Laboratories should note:

• The new version of GB/T 1.1-2020 to be released after 2020 has new requirements for the standard format.
• When the test results are close to the limit value, it is recommended to use ISO 11292:1995 for method comparison.