SN/T 4744-2017 in English

VALID

Determination of sexhormone residues of live animal for import and export一HPLC-MS/MS

Issued on:2017-05-12
Implemented on:2017-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

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Standard No:	SN/T 4744-2017
Document status:	VALID
Title in English:	Determination of sexhormone residues of live animal for import and export一HPLC-MS/MS
Title in Chinese:	进出口食用动物性激素残留量的测定液相色谱-质谱/质谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-05-12
Implemented on:	2017-12-01
Professional Classification:	SN-Import&Export Inspection
Related Topics:	laser mass spectrometry Second-hand liquid chromatography mass spectrometry How to Analyze Liquid Chromatography Mass Spectrometry How to Analyze Mass Spectrometry by Liquid Chromatography Second-hand liquid chromatography mass spectrometry No peak in liquid chromatography Liquid chromatography does not show High Quality Used Mass Spectrometry Quality of Used Mass Spectrometers Chromatographic coelution Imported gas chromatograph high performance mass spectrometry Stability Mass Spectrometry Mass Spectrometry Features Imported gas chromatograph liquid mass spectrometry No peak in mass spectrum Imported liquid phase Second-hand liquid chromatography mass spectrometry Imported liquid phase Mass Spectrometry Ionization Species Imported liquid chromatography Essence Spectrum Chromatographic coelution mass spectrometry hplc Essence Spectrum dynamic chromatography MS residue Determination of phenolphthalein and emodin in export health food by liquid chromatography-mass 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Introduction

Overview of detection method

This standard adopts high performance liquid chromatography-tandem mass spectrometry and solid phase extraction pretreatment technology to conduct qualitative and quantitative analysis of sex hormone residues in animal tissues. The main steps include:

Key instrument parameters

Chromatographic column type	Mobile phase composition	Mass spectrometry mode	Detection limit (μg/kg)
C18 column (1.7μm)	Methanol-acetonitrile gradient	MRM	0.5
NH2 column	Ammonium acetate buffer	ESI+/-	1.0

Technology evolution analysis

Compared to the traditional GC-MS method, the HPLC-MS/MS technology in this standard:

Increases detection sensitivity by 10 times
Shortens pre-treatment time by 40%
Can simultaneously detect steroidal and estrogen compounds

Actual application case

A beef exporting company used this standard for testing and found that the residues of methyltestosterone in two batches of samples were 1.2μg/kg and 0.8μg/kg, respectively. After optimizing the feed formula, the detection values in subsequent batches dropped to below 0.3μg/kg.

Implementation Recommendations

Laboratories should regularly verify the stability of the method using isotope internal standards such as testosterone-D3.
For high-fat samples, it is recommended to add a frozen centrifugation step.
Mass spectrometry parameters need to be fine-tuned according to the instrument model.

More samples: NB/T 47013.2-2015 YD/T 3934-2021 JJG 21-2008

Sample only — not a preview of SN/T 4744-2017

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