GB/T 13091-2018 in English

《GB/T 13091-2018饲料中沙门氏菌的测定》由TC76（全国饲料工业标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Determination of Salmonella in Feeds

1 Scope

This Standard specifies the general guidance on methods for the determination of Salmonella in feeds.

This Standard is applicable to the determination of Salmonella in feeds and feed additives.

2 Normative references

The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition (including any amendments) applies.

GB/T 6682 Water for analytical laboratory use—Specification and test methods

3 Culture media or materials

Except where indicated, reagents shall be of recognized analysis pure grade.

3.1 Water: It shall meet the requirements for Grade 3 water as specified in GB/T 6682.

3.2 Buffered peptone water (BPW): See B.1 of Appendix B.

3.3 Rappaport-Vassiliadis magnesium chloride-malachite green (RV) medium: See B.2 of Appendix B.

3.4 Selenite/cystine (SC) medium: See B.3 of Appendix B.

3.5 Bismuth sulfite (BS) agar: See B.4 of Appendix B.

3.6 Deoxycholate hydrogen sulfide lactose (DHL) agar: See B.5 of Appendix B.

3.7 Salmonella chromogenic medium.

3.8 Nutrient agar (NA): See B.6 of Appendix B.

3.9 Triple sugar iron (TSI) agar: See B.7 of Appendix B.

3.10 Peptone water and indole reagent: See B.8 of Appendix B.

3.11 Urea agar (pH 7.2): See B.9 of Appendix B.

3.12 Potassium cyanide (KCN) medium: See B.10 of Appendix B.

3.13 L-lysine decarboxylation medium: See B.11 of Appendix B.

3.14 Sugar fermentation tube: See B.12 of Appendix B.

3.15 O-nitrophenyl β-D-galactopyranoside (ONPG) medium: See B.13 of Appendix B.

3.16 Semi-solid agar: See B.14 of Appendix B.

3.17 Sodium malonate medium: See B.15 of Appendix B.

3.18 Salmonella factor О and H multivalent diagnostic serum, factor Vi diagnostic serum.

4 Apparatus

4.1 Refrigerator: 2°C–5°C.

4.2 Thermostatic incubators: 36°C ± 1°C, 42°C ± 1°C.

4.3 Homogenizer.

4.4 Oscillator.

4.5 Electronic balance: with a sensitivity of 0.1 g.

4.6 Aseptic conical flasks: with a volume of 500 mL, 250 mL; or aseptic homogenizingng bag.

Foreword i
1 Scope
2 Normative references
3 Culture media or materials
4 Apparatus
5 Sample
6 Test methods (See Appendix A for the diagram of test procedure)
7 Expression of results
Appendix A (Normative) Diagram of test procedure
Appendix B (Normative) Preparation and test method of culture media and reagents