GB/T 38571-2020 in English

Core analysis of standard technology

This standard uses Liquid chromatography-tandem mass spectrometry (LC-MS/MS) as the core detection equipment, and optimizes the extraction solvent system (acetonitrile-formic acid system) and mass spectrometry parameters to achieve simultaneous detection of 11 alkaloids. The method detection limit reaches 5-100ng/L, meeting the needs of trace analysis.

Key operation points

Mass spectrometry parameter optimization

The standard adopts positive ion mode (ESI+) and multiple reaction monitoring (MRM) technology, and sets differentiated parameters according to the characteristics of different alkaloids:

• Aconitine: select m/z 646→554 as the quantitative ion pair
• Strychnine: use m/z 335→184 to improve sensitivity
• The ion source temperature is uniformly set to 320℃ to ensure stability

Method Validation Data

Implementation suggestions

Things to note when implementing in the laboratory:

• Standard solutions should be stored at 4°C away from light (valid for 2 months)
• Regularly calibrate the mass axis of the mass spectrometer
• A blank test should be performed on each batch of samples