GB/T 40173-2021 in English

VALID

Determination of reducing-end sugar in water-soluble chitosan—Spectrophotometric method

Issued on:2021-05-21
Implemented on:2021-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $120.00
$117.00

Standard No:	GB/T 40173-2021
Document status:	VALID
Title in English:	Determination of reducing-end sugar in water-soluble chitosan—Spectrophotometric method
Title in Chinese:	水溶性壳聚糖中还原性端基糖的测定分光光度法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2021-05-21
Implemented on:	2021-12-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Keywords:	water-soluble chitosan spectrophotometric method introduction analysis water-soluble chitosan molecule method limit end sugar content method principle
Related Topics:	sugar Chitosan sugary Reducing sugar test Glycosyl reducing sugar in candy candy reducing sugar sugar concentration Restorative Water Solubility Concentration sugar determination reducing sugar in candy neutral base sugar fluorescent peak Determination of sugar content fluorescent sugar neutral base reducing sugar Total and Reducing Sugars end group reducing disaccharide Glycosylated Reducing sugars and total sugars Determination of reducing sugar in candy residual sugar Determination of reducing and total sugars The basis for the determination of reducing sugar Is total sugar the same as reducing sugar? total sugar reducing sugar Spectrophotometric measurement range Determination of total and reducing sugars Reducing sugar and total sugar Quantification of sugar Reducing sugars and total sugars Total and Reducing Sugars Determination of Nickel in Water by Atomic Spectrophotometry Spectrophotometer measuring water congenital glycosylation Reducing sugar + peak Method for Determination of Reducing Sugars Determination method of reducing sugar in foreign countries The main determination method of reducing sugar sugar calculation Determination of reducing sugar method Total sugars are more than reducing sugars 3. Reducing sugar Reducing sugar meter sugar reduction sugar reduction Glycosylated glycoforms Determination of sugar by ultraviolet spectrophotometry Method for Determination of Sugar Moisture glycosylated g2 Method for Determination of Total Reducing Sugars How to measure reducing sugar Photogenic Spectrophotometric measurement range Glycosylated glycoforms Glycation degree How to Measure Reducing Sugars Determination method of total sugar in water congenital glycosylation of reducing and total sugars Rapid Determination Method of Reducing Sugar soluble sugar reducing sugar How to measure reducing sugar? total sugar reducing sugar Determination method of reducing sugar and total sugar Determination of tin in water spectrophotometric method Sugar Chains and Glycosylation from reducing sugar Basic Principles of Photometry Pentose Reducing Determination of reducing sugar Others Method for Determination of Brix GBT40173 GB/T 40173-2021 Hydroxypropyl Chitosan Nitrile reduction Chitosan solubility Solubility of chitosan in acid Determination principle of reducing sugar in beverages b3gnt glycosylation sting glycosylation dtt solution Chitosan solubility Chitosan solubility Glycosylation of sirt1 sirt1 glycosylation How to measure fructose by spectrophotometric techniques The principle of measuring reducing sugar by potassium ferricyanide method Disadvantages of the potassium ferricyanide method for determining reducing sugars Calculation of reducing sugars determined by direct titration method Spectrophotometric determination of saccharin sodium in pastries Spectrophotometer testing water-soluble fluorine standard curve Principles and methods of detecting sugar Determination method of reducing sugar in corn juice What are the methods for measuring reducing sugars? Method for determining the presence of reducing sugars Quantitative method for reducing sugars Quantitative determination method of reducing sugars Please help me determine the total sugar in sweet and sour pickled vegetables. The most suitable method for measuring aldose reducing sugars Rapid method for determination of reducing sugars How to titrate reducing sugars Polysaccharide qualitative method Method steps for measuring reducing sugars Determination method of total reducing sugars Three methods for determining reducing sugars Determination method of crude chitosan concentration General method for determination of reducing sugars Spectrophotometric dextran detection method Soluble sugar test method How to measure reducing sugars Reducing sugar determination method direct titration method Methods to identify reducing properties of sugars Method for determination of moisture content of insoluble saccharin Standard method for determination of soluble sugars Soluble sugar determination method Reducing sugar filtering and drying method What are the methods for measuring soluble sugar? Method for hydrolyzing protopectin into reducing sugar Scleroglucan spectrophotometer End group determination What are the methods for determining reducing sugar? gbt40173

《GB/T 40173-2021水溶性壳聚糖中还原性端基糖的测定分光光度法》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Analysis of the core content of the standard

This standard uses a spectrophotometer to determine the reducing end sugar content at the end of the water-soluble chitosan molecule, and establishes a quantitative relationship at 590nm through the MBTH-Fe³⁺ color development system.

Key technical indicators

Parameters	Indicator requirements	Detection significance
Detection limit	0.006mmol/g	Reflects the sensitivity of the method
Limit of quantification	0.023mmol/g	Ensure reliable quantitative range
Precision	RSD≤10%	Ensure the repeatability of the results

Detailed explanation of the method principle

Based on a three-step color reaction:
1. MBTH reacts with reducing sugars to form azine under alkaline conditions
2. Fe³⁺ oxidizes excess MBTH to form cations
3. The cyan-blue compound produces characteristic absorption at 590nm

Key control points: 80℃ water bath for 13min requires precise control, and the color should be measured immediately after 1h of development.

Implementation recommendations

The standard solution should be prepared and used immediately. The MBTH reagent is valid for only 10 days.
The temperature deviation needs to be calibrated when using a constant temperature water bath.
After the sample is dissolved, it needs to be left to stand and the supernatant should be taken to avoid turbidity interference.
It is recommended to carry a blank control and a quality control sample for each batch of measurements.

Technology evolution analysis

Compared with the traditional DNS method, this method has the following advantages:
- Better selectivity (not interfered by free amino groups)
- Sensitivity increased by 3 times
- Color stability extended to 2 hours

More samples: JGJ 94-2008 GB 18306-2015 NB/T 47013.2-2015

Sample only — not a preview of GB/T 40173-2021

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

GB/Z 260-2026 in English

Biomimetics—Ontology-Enhanced Thesaurus (OET) for biomimetics
2026-08-27
GB/T 47142-2026 in English

Determination of molecular weight of polysaccharides—Size exclusion chromatography with multi-angler laser light scattering method
2026-01-28
GB/T 33681.1-2017 in English

Methods to prepare samples for high-throughput gene sequencing—Part 1：Preparing samples of animal tissues
2017-05-12
GB/T 42076.2-2025 in English

Biotechnology—Cell Counting—Part 2: Experimental design and statistical analysis to quantify counting method performance
2025-10-31