SN/T 5334.3-2020 in English

VALID

Protocol of digital PCR for quantitatively detecting genetically modified plants and their derived products - Part 3: Genetically modified maize

Issued on:2020-12-30
Implemented on:2021-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $130.00
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Standard No:	SN/T 5334.3-2020
Document status:	VALID
Title in English:	Protocol of digital PCR for quantitatively detecting genetically modified plants and their derived products - Part 3: Genetically modified maize
Title in Chinese:	转基因植物产品的数字PCR检测方法第3部分：转基因玉米
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-12-30
Implemented on:	2021-07-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	digital pcr digital pcr platform technical frameworks dimensions traditional qpcr digital pcr detection principle dependence maize introduction interpretation maize hmg gene
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Introduction

Interpretation of the core content of the standard

As an industry standard implemented by the General Administration of Customs in 2021, this standard establishes a quantitative detection system for genetically modified corn based on the digital PCR platform, focusing on detecting 10 varieties including 98140 and DAS40278-9.

Comparison of technical frameworks

Dimensions	Traditional qPCR	Digital PCR
Detection principle	Dependence on standard curve	Absolute quantification
Sensitivity	0.1%	0.01%
Anti-interference ability	Susceptible to inhibitors	Strong tolerance

Key technical parameters

The standard stipulates that the maize HMG gene should be used as the internal reference, and the reaction system should be:

Template DNA dosage: 60ng
Probe labeling: FAM/BHQ-1 (strain), HEX/BHQ-1 (internal reference)
Annealing temperature: For strain differences, see Appendix A (mainly 60℃)

Analysis of implementation difficulties

Take the BVLA430101 strain as an example:

Special attention should be paid to the design of the 3' end cross-border sequence
The probe concentration needs to be adjusted to 250nM (different from the conventional 180nM)
The detection limit reaches 3 copies/μL (quantitative)

Background of standard evolution

Main updates of the 2020 version:

Added detection methods for 4 strains including MON87427
Optimized droplet generation parameters
Clarified post-processing requirements for different platforms

More samples: DL/T 368-2010 JC/T 2453-2018 JJG 21-2008

Sample only — not a preview of SN/T 5334.3-2020

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