SN/T 5334.4-2020 in English

VALID

Protocol of digital PCR for quantitatively detecting genetically modified plants and their derived products - Part 4: Genetically modified canola

Issued on:2020-12-30
Implemented on:2021-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	SN/T 5334.4-2020
Document status:	VALID
Title in English:	Protocol of digital PCR for quantitatively detecting genetically modified plants and their derived products - Part 4: Genetically modified canola
Title in Chinese:	转基因植物产品的数字PCR检测方法第4部分：转基因油菜
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-12-30
Implemented on:	2021-07-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	digital pcr droplet digital pcr technology fourth part standard dpcr detection sensitivity canola introduction analysis
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Introduction

Analysis of the core content of the standard

As the fourth part of the SN/T 5334 series, this standard specifically establishes a digital PCR quantitative detection system for genetically modified rapeseed. The technical points include:

Detection dimension	Traditional qPCR	This standard dPCR
Detection sensitivity	0.1%	0.01%
Absolute quantification	Standard curve required	Direct counting
Anti-inhibition ability	Susceptible to interference	Strong tolerance

Key technical parameters

The standard clearly stipulates class=instrument>PEP internal reference gene and 11 exogenous genes detection requirements:

Reaction system: Dual channel/single channel configuration (see Table 1-2)
Template amount: 60ng DNA
Annealing temperature: 56-60℃ according to strain differences (Appendix A)

Implementation points

Case: Single channel mode is required for detecting MS8 strains, probe concentration is 180nM, and annealing temperature is 56℃ to obtain the best separation.

For the special strain 73496, it is necessary to pay attention to the difference between its probe labeling method (5'FAM/3'MGBNFQ) and other strains.

Analysis of Standard Evolution

Compared with the 2015 version of the qPCR method, this standard has the following major breakthroughs:

Introduction of Droplet Digital PCR technology to improve detection accuracy
Added detection schemes for 4 newly approved strains including Topas19/2
Unified reaction procedures for different platforms (thermal cycling steps cannot be modified)

More samples: JGJ 94-2008 GB 18306-2015 JJG 21-2008

Sample only — not a preview of SN/T 5334.4-2020

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