GB/T 33752-2017 in English
VALIDAldehyde slide for microarrays
- Issued on:2017-05-12
- Implemented on:2017-12-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$107.00
《GB/T 33752-2017微阵列芯片用醛基基片》由TC421(全国生物芯片标准化技术委员会)归口,主管部门为国家标准化管理委员会。
Introduction
Interpretation of the National Standard of the People's Republic of China GB/T 33752—2017
| Standard dimensions | Technical requirements | Test methods | Compliance recommendations |
|---|---|---|---|
| Appearance and dimensions | - No particles or obvious scratches within the effective sampling range- Specifications can be customized, such as rectangle (length, width and thickness: 75.5mm±0.2mm × 25.2mm±0.2mm × 1.00mm±0.05mm) | Visual inspection and vernier caliper measurement | Regular sampling is recommended to ensure dimensional accuracy |
| Surface hydrophobicity | - Contact angle range: 30°~70° - Coefficient of variation (CV) ≤20% | Use an optical contact angle meter to test 6 points and take the average value | Optimize surface treatment process to ensure consistency |
| Fixation uniformity | - Fluorescence signal intensity variation ≤25% | Use a laser confocal scanner for scanning test | Select high-quality probes to improve fixation efficiency |
Analysis of standard formulation background and technological evolution
Since its development at the end of the last century, microarray chip technology has been widely used in gene expression analysis, protein interaction research and other fields. With the advancement of biotechnology, higher requirements are put forward for substrate performance:
- Material improvement: Gradually shift from early non-specific surfaces to functional modification, such as introducing aldehyde groups to enhance molecular fixation ability.
- Manufacturing process improvement: Dimensional accuracy moves from millimeter level to micrometer level to meet the needs of high-density chips.
- Detection method innovation: From simple visual inspection to optical contact angle measurement and fluorescence scanning technology.
Implementation suggestions
To ensure the effective implementation of GB/T 33752—2017, the following suggestions are put forward:
- Training: Regularly conduct standard interpretation and operation specification training for relevant personnel.
- Equipment calibration: Ensure the accuracy and stability of measuring instruments (such as vernier calipers, contact angle meters).
- Quality Control: Establish a full-process quality monitoring system from raw material procurement to finished product delivery.
- Continuous Improvement: Optimize production processes and technical parameters based on actual usage feedback.

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