SN/T 5147-2019 in English

VALID

Determination of inositol in milk products LC-MS/MS method for export

Issued on:2019-10-25
Implemented on:2020-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $100.00
$97.00

Standard No:	SN/T 5147-2019
Document status:	VALID
Title in English:	Determination of inositol in milk products LC-MS/MS method for export
Title in Chinese:	出口乳制品中肌醇的测定液相色谱-质谱/质谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-10-25
Implemented on:	2020-05-01
Professional Classification:	SN-Import&Export Inspection
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Introduction

Analysis of Standard Technical Framework

Core Elements	Technical Requirements	Method Characteristics
Detection Principle	Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	Using Alantis T3 chromatographic column, ESI ion source
Limit of Quantitation	0.01mg/kg	Meets the requirements of EU Regulation EU 1169/2011
Recovery Range	90%-110%	3 added concentrations for verification (10/2/25mg/kg)

Key Operation Points

Sample Pretreatment: Weigh 2.5g of sample and extract it in a 40-50℃ water bath, then centrifuge at 12000r/min for 20 minutes and filter through a 0.45μm filter. Add isotope internal standard D6-inositol for calibration.

Chromatographic Conditions: The mobile phase is 0.2% formic acid aqueous solution-methanol (2+98), the flow rate is 0.20mL/min, the column temperature is 40℃, and the injection volume is 10μL.

Mass spectrometry parameter optimization

Ion pair: Inositol m/z 179.1→161.0 (quantitative ion), 185.0→166.9 (internal standard ion)
Collision energy: Optimization in the range of -15V to -20V
Ion source parameters: ESI negative ion mode, nebulizer gas (N2) pressure 50psi

Implementation suggestions

The standard solution should be prepared and used immediately and stored at 4℃ for no more than 3 days
Blank test and spike recovery should be carried out simultaneously for each batch of samples
It is recommended to use a triple quadrupole mass spectrometer such as AB Sciex 4000 QTRAP

More samples: NB/T 47013.2-2015 QC/T 942-2021 GB 18306-2015

Sample only — not a preview of SN/T 5147-2019

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