SN/T 5451-2022 in English

VALID

Commercial kit method - Lactic acid bacteria count - Test method I

Issued on:2022-03-14
Implemented on:2022-10-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $210.00
$204.00

Standard No:	SN/T 5451-2022
Document status:	VALID
Title in English:	Commercial kit method - Lactic acid bacteria count - Test method I
Title in Chinese:	商品化试剂盒检测方法乳酸菌总数方法一
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2022-03-14
Implemented on:	2022-10-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	test method test plate petrifilm lactic acid bacteria test plates test plate method pretreatment time ≥30min ≤15min cultivation period analysis dimensions traditional plate method
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Introduction

Analysis of the core content of the standard

This standard specifies the application specifications of 3M Petrifilm Lactic Acid Bacteria Test Plates in food microbiological testing. The main technical features include:

Integrated culture system: the test plate contains culture medium, selection agent and tetrazolium indicator
Two-phase detection principle: quantitative analysis is achieved through red colony color development and aerobic culture
Multi-genus compatibility: Lactobacillus, Bifidobacterium and Thermophilic Streptococcus can be detected

Method comparison and analysis

Dimensions	Traditional plate method (GB 4789.35)	3M test plate method
Pretreatment time	≥30min	≤15min
Cultivation period	72h	48h±3h
Result interpretation	Need colony isolation and identification	Direct colorimetric counting
Space occupation	Large incubator required	Can stack 20 pieces/layer

Key operating points

Sample processing specifications

Differences in processing for different sample types:

Solid sample: 25g+225mL buffer, homogenize for 1-2min
Liquid sample: Pre-set glass beads and shake to mix
Frozen sample: Thaw at 2-5℃≤18h or thaw quickly at 45℃

Precautions for culture

Typical problem handling in the use of test pieces:

Gel liquefaction phenomenon: Count the non-liquefied area to estimate the total amount
Too dense colonies: Select representative grids for estimation (30 grid system)
Edge diffusion: Use 3M pressure plate to ensure uniform distribution of sample liquid

Interpretation of verification data

According to the verification results in Appendix A:

Linear relationship: Correlation coefficient R²＞0.98 (8 matrices including pickled pepper chicken feet)
Precision: RSD≤3.5% (high and low concentration samples)
Density resistance: stable results within the range of 35-37℃, 45-51h

Implementation suggestions

Establish test piece acceptance criteria: verify batch number consistency (see Appendix A.3)
Complex samples need to be supplemented with GB 4789.35
Periodically compare methods: it is recommended to perform a traditional plate method control once a quarter

More samples: QC/T 942-2021 DL/T 368-2010 YD/T 3934-2021

Sample only — not a preview of SN/T 5451-2022

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