SN/T 4547-2017 in English

VALID

Commercial kit method-Coliforms and escherichia coli-Test method Ⅰ

Issued on:2017-05-12
Implemented on:2017-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $200.00
$194.00

Standard No:	SN/T 4547-2017
Document status:	VALID
Title in English:	Commercial kit method-Coliforms and escherichia coli-Test method Ⅰ
Title in Chinese:	商品化试剂盒检测方法大肠菌群和大肠杆菌方法一
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-05-12
Implemented on:	2017-12-01
Superseding:	SN/T 1896-2007 Rapid enumeration of coliforms and Escherichia coli in foods - Petrifilm TM coliform count plate and Petrifilm TM E.coli/coliform count plate method
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	foods-petrifilm™ test plate method commercial kit detection method detection method mpn method traditional method repeatability test method principle
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Introduction

Core Changes of the Standard Revision

Comparison Dimensions	SN/T 1896-2007	SN/T 4547-2017
Standard Name	Rapid Count of Coliforms and Escherichia coli in Foods-Petrifilm™ Test Plate Method	Commercial Kit Detection Method for Coliforms and Escherichia coli-Method 1
Detection Method	Includes the MPN Method for Petrifilm™ Test Plates	Deletes the MPN Method, and retains the Plate Count Method
Technical Parameters	Unclear calculation formula	New colony calculation formula (Formula 1)

Detailed explanation of the method principle

The test piece adopts a double-layer membrane structure design, and the lower membrane is pre-prepared with VRB culture medium and β-glucuronidase indicator:

Escherichia coli determination: β-glucuronidase decomposes the indicator, forming blue colonies with bubbles
Coliform group determination: Fermentation of lactose produces acid, causing the pH indicator to turn red, forming red colonies with bubbles

Typical application case

A poultry processing company uses 3M Petrifilm™ E. coli/Coliform test piece to detect thawed chicken:

After 24 hours of incubation, 12 blue colonies (E. coli) and 25 red colonies (Coliform bacteria) appeared on the test piece.
According to the formula, the number of E. coli was calculated to be 1.2×10² CFU/g, which meets the limit requirements of GB 16869-2005.

Key Operation Points

Operation Steps	Technical Requirements	Common Errors
Sample Preparation	Homogenization time 1-2min, pH adjusted to 6.5-7.5	Thaw temperature exceeding 45℃ will cause the death of microorganisms
Inoculation Operation	1mL sample solution is added dropwise vertically, and the pressing plate is left to stand for ≥1min.	Bubble generation affects colony diffusion.
Culture conditions	36±1℃, stacking ≤20 plates.	Temperature fluctuation leads to abnormal color development.

Implementation recommendations

Key points of quality control

A blank control (phosphate buffer) is required for each batch of testing.
It is recommended to use a slap homogenizer for highly contaminated samples.
The incubator temperature needs to be calibrated and recorded daily.

Method validation requirements

When used in the laboratory for the first time:

Compare with GB Comparison test of 4789.3 traditional method
Repeatability test of different operators (RSD≤15%)
Storage stability verification of test pieces (stored at 2-8℃)

More samples: GB 18306-2015 DL/T 5154-2012 QC/T 942-2021

Sample only — not a preview of SN/T 4547-2017

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