SN/T 5140-2019 in English

VALID

Determination of sulfonamides residues in foodstuffs of animal origin for export

Issued on:2019-10-25
Implemented on:2020-05-01
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $540.00
$524.00

Standard No:	SN/T 5140-2019
Document status:	VALID
Title in English:	Determination of sulfonamides residues in foodstuffs of animal origin for export
Title in Chinese:	出口动物源食品中磺胺类药物残留量的测定
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2019-10-25
Implemented on:	2020-05-01
Superseding:	SN 0498-1995 Method for the determination of sulfadimethoxine residues in meat for export SN/T 1965-2007 Determination of sulfonamides residues in eels and eel products - High performance liquid chromatographic method SN/T 2580-2010 Determination of Sixteen Sulfonamides Residues in Royal Jelly for Import and Export - HPLC-MS/MS
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	animal origin lc-ms/ms method applicable matrix livestock lc-ms/ms method animal fat export introduction technical evolution
Related Topics:	animal source drug residue drug in english export medicine Sulfonamides Sulfamethizole animal sulfonamides food sulfa Drugs of animal origin sulfa drug animals drug residue Pesticide residues in foods of plant origin Sulfonamides Sulfonamides Sulfa liquid phase Sulfa Many residues of 13 sulfa drugs in animal food Drugs of animal origin animal export Food and Drug Residues of Animal Origin animal sulfa drugs animal source food residue ms Sixteen sulfa drug residues Sulfa in food Sixteen sulfa drugs residues Sulfa in animal food Monitoring of Residues in Exported Foods of Animal Origin Liquid phase of sulfonamides Sixteen kinds of sulfonamide drug residues in livestock and poultry meat sulfa drugs in which foods sulfonamides Sulfa mass spectrometry Food sulfa drugs of animal origin small animal food Many residues of glucocorticoids in food of animal origin Sulfa mass spectrometry Food sulfa drugs of animal origin Five kinds of sulfur in food of animal origin Substance residues in food residues in food export sulfur five types of food Sulfa drugs for animals sulfur export animal sulfa drugs food sulfa drugs Also for the detection of sulfa drugs Domestic drug export Food Thirteen Sulfonamides organic amine food Export monitoring of animal-derived residues Drug export k in medicine Drug export copper in animal food Sulfur food export food Multi-residue detection of glucocorticoids in food of animal origin How long do sulfa drugs last? 19 Nortestosterone residues in food of animal origin for import and export A method for detecting drug residues in food of animal origin Edible sulfur polyamine food polyamine food Drug residues in eggs lithium in food export medicine organic food export Food Automatic Detention food exports by organic food export export organic food amine food Residues of progesterone drugs in food of animal origin for import and export Class 3 drugs export organic food Kidney determination of animal food Food exports to Central Asia Sulfonamide Veterinary Drugs food of animal origin Reactions between drugs and leachables Chinese medicine in food adc drug linker type Content of residual solvent methanesulfonic acid in raw materials Sulfonamide antibiotic pesticide residues Structure of commonly used sulfa drugs Sulfonamide residues Sulfonamide residues in dairy products Sulfonamide residues in milk Sulfa Drug Residues International Nafil residues in food Veterinary drug residues in feeds of animal origin Acetaldehyde residues in medicines Sulfa drug regulation Ethylene oxide remains in food and drugs Sulfonamides in the environment How to identify sulfa drugs Validation of cleaning methods for sulfonamides

Introduction

Technical evolution and background of standards

This standard integrates and replaces the three old standards SN 0498-1995, SN/T 1965-2007 and SN/T 2580-2010. The main technical improvements include:

Detection range expansion: HPLC method adds livestock and poultry muscle matrix and 9 sulfonamide drugs (sulfathiazole, sulfapyridine, etc.)
Method upgrade: LC-MS/MS method adds honey/fat matrix and 2 drugs sulfacetamide and sulfachloropyridine
Technology integration: unified pretreatment methods for different matrices, optimized chromatographic separation conditions

Comparison of core detection methods

Dimensions	HPLC method	LC-MS/MS method
Applicable matrix	Livestock and poultry muscle, aquatic products	Muscle/liver/fat/egg/milk/honey/royal jelly
Detection drugs	17 kinds	19 kinds (sulfacetamide and sulfachloropyridine were newly added)
Detection limit (μg/kg)	10	2.0 (bee products 5.0)
Quantification method	External standard method	Isotope internal standard method

Key operating points

Pretreatment process optimization

HPLC method adopts ethyl acetate extraction → n-hexane defatting → MCX column purification; LC-MS/MS method adds a trichloroacetic acid solution extraction step to the fat matrix, and the royal jelly needs to be purified twice by C18 column.

Instrument parameter setting

HPLC conditions: C18 column (4.6×250mm, 5μm), gradient elution (methanol/0.3% acetic acid-2mM ammonium acetate), detection wavelength 268nm
LC-MS/MS conditions: ESI positive ion mode, multiple reaction monitoring (MRM), see Appendix D for specific collision energy

Implementation suggestions

For high-fat samples (such as animal fat), it is recommended to use the LC-MS/MS method to ensure the recovery rate (85-115%)
When detecting bee products, special attention should be paid to the matrix effect, and the use of isotope internal standard compensation is recommended
Regularly verify the performance of the solid phase extraction column, and the methanol activation volume should be no less than 5mL

Technical indicator verification

Typical recovery data (pork matrix):

Sulfadiazine: 75.4-90.0% (10μg/kg spike level)
Sulfadimethoxine: 91.2-116.3% (20μg/kg)
Sulfaquinoxaline: 89.5-114.3% (200μg/kg)

More samples: JGJ 94-2008 JJG 21-2008 DL/T 368-2010

Sample only — not a preview of SN/T 5140-2019

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