SN/T 5359-2021 in English

VALID

Determination of azithromycin residue in foodstuffs of animal origin for export-LC-MS/MS method

Issued on:2021-11-22
Implemented on:2022-06-01
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $500.00
$485.00

Standard No:	SN/T 5359-2021
Document status:	VALID
Title in English:	Determination of azithromycin residue in foodstuffs of animal origin for export-LC-MS/MS method
Title in Chinese:	出口动物源食品中阿奇霉素残留量的测定液相色谱―质谱/质谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2021-11-22
Implemented on:	2022-06-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	export-lc-ms/ms method introduction analysis method detection range matrix deposition method validation elisa method animal origin
Related Topics:	liquid chromatography mass spectrometry Liquid chromatography mass spectrometry· odd column Liquid Chromatography-Mass Spectrometry/Mass Spectrometry How to Analyze Mass Spectrometry by Liquid Chromatography Mass Spectrometry Products No peak in liquid chromatography Liquid chromatography does not show Chromatographic coelution liquid chromatography mass spectrometry Mass Spectrometry Products Spectrum Liquid Chromatography-Mass Spectrometry liquid mass spectrometry No peak in mass spectrum Mass Spectrometry Conditions liquid chromatography mass spectrometry Liquid chromatography + mass spectrometry Liquid Chromatography + Mass Spectrometry Essence Spectrum Chromatographic coelution mass spectrometry hplc Mass Spectrometry Conditions Essence Spectrum MS residue Determination of phenolphthalein and emodin in export health food by liquid chromatography-mass spectrometry/mass spectrometry Spectro-Mass Spectrometry No peak in mass spectrum mass spectrometry semi-quantitative semi-quantitative mass spectrometry No peak in liquid chromatography Liquid Chromatography-Mass Spectrometry/Mass Spectrometry quantitative mass spectrometry Liquid Chromatography Mass Spectrometry/Mass Spectrometry Retention time of gas chromatography mass spectrometry Quantity measured by mass spectrometry Liquid Chromatography Mass Spectrometry/Mass Spectrometry Liquid Chromatography Mass Spectrometry/Mass Spectrometry no chromatographic peak Chromatographic peak Chromatographic peak mass spectrometry semi-quantitative Substance mobile phase semi-quantitative mass spectrometry Coeluting peaks Mass Spectrometry deuterated internal standard Determination of pymetrozine residues in food for export by liquid chromatography-mass spectrometry/mass spectrometry mass spectrum liquid mass spectrometry No peak in gas chromatography mass spectrometry GC mass spectrometry does not produce peaks from time to time liquid mass spectrometry membrane mass spectrometry Liquid Chromatography-Mass Spectrometry/Mass Spectrometry Spectrum material neutral mass spectrum MS mass 18 liquid chromatography mass spectrometry mass spectrometry Liquid Chromatography-Mass Spectrometry Mass Spectrometry Liquid Chromatography/Mass Spectrometry Liquid Chromatography - Mass Spectrometry mass spectrometry Liquid Chromatography + Mass Spectrometry + Chromatographic round peak MS co-elution Chromatographic Dynamics Mass spectrometry source LC-MS co-elution Liquid chromatography + peak extraction co-elution chromatography Liquid chromatography peaks within a few minutes at the earliest Mass Spectrometry Food Testing Liquid chromatography peaks in 70 minutes mass spectrometry animal export Gas source for mass spectrometry mass spectrum peak Pinsheng mass spectrometry Pinsheng, mass spectrometry Detection of 11 hormone residues in food of animal origin by liquid chromatography-mass spectrometry Penicillin antibiotics in food of animal origin MS effluent Normal phase chromatography + peak extraction Liquid chromatography peaks in one minute Quantification by mass spectrometry Effluent liquid phase liquid residue LC peaks prematurely mass spectrometry Food Inspection Liquid Chromatography Mass Spectrometry MS co-elution Monitoring of Residues in Exported Foods of Animal Origin Terms in Mass Spectrometry Mass Spectrometry Liquid Chromatography Peak Quantification by liquid chromatography mass spectrometry liquid phase MS residue The mass spectrum has very small peaks Absolute quantitation by mass spectrometry Foods of Animal Origin Liquid Chromatography mass spectrometry LC outlet ring eluting from the chromatogram Mass Spectrometry Food Testing mass spectrometry Amphotericin Mass Spectrometry spectinomycin Mass Spectrometry Internal Quantification Substance residues in food residues in food liquid-mass spectrometry No peak in normal phase chromatography Mass Spectrometry + Determination + Quantity Liquid chromatography + chromatography + mass spectrometry liquid chromatography mass spectrometry mass spectrometry liquid measurable substance Liquid Chromatography + Mass Spectrometry + Mass Spectrometry Absolute quantitation by mass spectrometry Chromatographic peaks co-elute MS/MS chromatography substance Liquid chromatography mass spectrometry+ deuterated internal standard Odd Spectrum Mass spectrometry + semi-quantitative post elution chromatography Liquid Chromatography + Mass Spectrometry A chromatographic peak What is Food Grade Coloring Substance? Liquid chromatography + mass spectrometry + quantification Mass Spectrometry Food Testing Spectrum+ Spectrum magnetic mass spectrometry Liquid Chromatography Peak Front Export monitoring of animal-derived residues Quantitative mass spectrometry Mass Spectrum 41 Peak liquid chromatography mass spectrometry food Chromatographic mass fraction mass spectrometry spectrum MS mass 36 The liquid chromatography had a peak last time and no peak this time liquid chromatography mass spectrometry Liquid Chromatography - Mass Spectrometry Residues of progesterone drugs in food of animal origin for import and export Liquid chromatography-mass spectrometry quantification Mass Spectrometry + Source Chromatographic peak Chromatography of animal origin food of animal origin Residues of virginiamycin in foods of animal origin Negative peaks appear in the chromatogram HPLC peak time Mobile phase for mass spectrometry Mobile phase outlet Liquid chromatography mass spectrometry APCI source National standard food pigment liquid phase Mass spectrum animation Mass Spectrometry Method EI Source Compounds with no peaks in the mass spectrum Three methods for determining the content of azithromycin Azithromycin content detection method HPLC column packing quality Acidic substances produce double peaks in liquid phase method The liquid mass method did not produce peaks. Mass spectrometry parameters in quality standards LC column particle size Fosfomycin calcium mass spectrometry method Liquid chromatography mass spectrometry gold Mass spectrometry power supply stability test Azithromycin content determination standard chart Azithromycin content determination standard chart Azithromycin detection method and HPLC column quality

Introduction

Analysis of the core content of the standard

Technical elements	Requirements of SN/T 5359-2021	Advantages of the method
Detection range	11 types of animal-derived matrices (chicken, honey, casings, etc.)	Covering major edible animal products
Limit of quantification	1.0μg/kg	Meet EU MRL requirements (50μg/kg)
Recovery rate	85.4%-109%	Meets the requirements of GB/T 27404
Analysis time	10min/sample	3 times more efficient than traditional methods

Key technical points

Innovation in sample pretreatment: The composite purification strategy of PSA adsorbent and n-hexane defatting is used to effectively solve the complex matrix problems of animal-derived samples. For example, honey samples need to be melted in a 60℃ water bath before testing to ensure homogeneity.

Mass spectrometry parameter optimization: m/z 749.5→591.3 was selected as the quantitative ion pair and azithromycin-D5 (m/z 754.5) was used as the internal standard to compensate for the signal suppression caused by the matrix effect (average correction efficiency 92-107%).

Implementation and application recommendations

Quality control:Each batch of samples should be accompanied by blank controls and spike recovery experiments, and the recovery rate should be in the range of 80-110%
Instrument maintenance:It is recommended to clean the ESI ion source after every 50 injections to prevent matrix deposition
Method validation:When changing the brand of chromatographic column, the mobile phase gradient needs to be re-optimized (see standard Table 1)

Standard evolution analysis

Compared with the ELISA method of SN/T 2316-2009, the LC-MS/MS technology of this standard increases the specificity to 99.7% and reduces the false positive rate to 0.3%. The 2021 version adds validation data for royal jelly and casing matrices, expanding the applicability of the standard.

More samples: GB 50021-2001(2009) YD/T 3934-2021 DL/T 5154-2012

Sample only — not a preview of SN/T 5359-2021

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