GB/T 38086-2019 in English

1. Standard Overview

GB/T 38086-2019 specifies the method for determining the content of rabbit metallothionein (MT-I and MT-Ⅱ) in biological samples using liquid chromatography-tandem mass spectrometry. This method is suitable for the detection of metallothionein in biological preparations or extracts, with a detection limit of 6.2~mg/kg.

2. Technical Interpretation and Implementation Guide

2.1 Selection and synthesis of characteristic peptides

The characteristic peptide CAQGCICK (labeled as LCAM) was used to detect MT-I and MT-II. The relative molecular mass of the peptide is 995, the purity is over 95%, and the appearance is white powder.

2.2 Quantitative and qualitative analysis

The parent ion m/z 498.7 and the daughter ion m/z 637.3 were selected for quantification by liquid chromatography and tandem mass spectrometry. The qualitative judgment was based on the maximum allowable deviation of retention time and relative ion abundance (Table 1).

3. Implementation Suggestions

To ensure the effective implementation of the standard, it is recommended that:

• Use reagents and instruments that meet the requirements.
• Strictly follow the enzymatic hydrolysis and sample preparation steps.
• Regularly calibrate the instrument and perform blank tests to verify the accuracy of the method.
• Ensure the recovery rate is within the range of 80%~120% in the spike recovery test.