High performance liquid chromatography detection room

High performance liquid chromatography detection room, Total:200 items.

The international standard classification for "High performance liquid chromatography detection room" includes: Protection against crime , Beekeeping , Chemical analysis , Other standards related to optics and optical measurements , food products in general , Textiles in general , Fruits. Vegetables and derived products in general , Meat, meat products and other animal produce , Cosmetics. Toiletries , Biology. Botany. Zoology , Workplace atmospheres , Cereals, pulses and derived products , Animal and vegetable fats and oils , Farming and forestry in general , Animal husbandry and breeding , Oilseeds , General methods of tests and analysis for food products , Textile fabrics , Animal feeding stuffs .

The Chinese standard classification for "High performance liquid chromatography detection room" includes: Crime judging technology , Beekeeping, silkworm keeping , Basic standards and general methods , chromatograph , Basic standards and general methods , Cash crop in general , Cosmetics , Environmental Condition and General Test Method , Labor sanitation , Cereal crop and forage crop in general , Food processing and products in general , Grease and oil processing and products , Basic standards and general methods , Livestock and poultry product , Oil crop and product , Fuel oil , Basic standards and general methods , Basic standards and general methods , Livestock and poultry feed and additive .

Professional Standard - Agriculture

20兽药残留检测标准操作规程-2008 Determination method of sulfonamide drug residues in porcine muscle-high performance liquid chromatography
28兽药残留检测标准操作规程-2008 Method for detection of ceftiofur residues in bovine and porcine tissues--high performance liquid chromatography
35兽药残留检测标准操作规程-2008 Detection method of chloramphenicol residues in pig liver tissue--high performance liquid chromatography-tandem mass spectrometry
2306药典二部-2010 Appendix V D High Performance Liquid Chromatography
26兽药残留检测标准操作规程-2008 Determination method of tilmicosin residues in milk-high performance liquid chromatography
158药典三部-2010 Appendix III Chromatography IIIB High Performance Liquid Chromatography
183药典三部-2015 Chromatography 0512 General Principles of High Performance Liquid Chromatography 23
NY/T 2895-2016 Determination of folic acid in feeds. High performance liquid chromatography
NY/T 1381-2007 Determination of Capsaicin by High Performance Liquid Chromatography
63药典四部-2020 0512 High performance liquid chromatography
27兽药残留检测标准操作规程-2008 Determination method of ceftiofur residues in milk-high performance liquid chromatography
782兽药典二部-2015 Appendix Contents 0500 Chromatography 0512 High Performance Liquid Chromatography
NY/T 3164-2017 Determination of anthocyanins in black rice-High-performance liquid chromatography
1766药典一部-2010 Appendix VI D High Performance Liquid Chromatography
40兽药残留检测标准操作规程-2008 Determination method of pyrilimycin residues in milk-high performance liquid chromatography-tandem mass spectrometry
789兽药典一部-2015 Appendix Contents 0500 Chromatography 0512 High Performance Liquid Chromatography
82药典四部-2015 0512 High performance liquid chromatography
202药典三部-2020 Chromatography 0512 High Performance Liquid Chromatography

Professional Standard - Public Safety Standards

GA/T 1513-2018 Forensic science—Examination methods for stamp traces—High performance liquid chromatography

Guangxi Provincial Standard of the People's Republic of China

DB45/T 1153-2015 High performance liquid chromatography for the detection of sucrose content in intermediate products in the sugar production process

National Metrological Verification Regulations of the People's Republic of China

JJG(教委) 024-1996 Verification Regulations for High Performance Liquid Chromatography

Chongqing Provincial Food Standard of the People's Republic of China

DBS50/ 015-2013 High performance liquid chromatography for the detection of red powder in food

Professional Standard - Supply and Marketing Cooperative

GH/T 1081-2012 Determination of poplar tree gum in propolis—Reversed-phase high performance liquid chromatography method

General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China

GB/T 24903-2010 Inspection of grain and oils—Determination of resveratrol in peanut by high performance liquid chromatography
GB/T 40833-2021 Determination of p-coumaric acid in sugarcane rinds—High performance liquid chromatography
GB/T 29664-2013 Determination of vitamin B3(nicotinic acid and nicotinamide)in cosmetics—HPLC and HPLC-MS/MS
GB/T 16631-2008 General rules for high performance liquid chromatography
GB/T 20362-2006 Method for the determination of clopidol residues in eggs - High performance liquid chromatographic
GB/T 25221-2010 Inspection of grain and oils—Determination of ergosterol in grain—Normal phase high performance liquid chromatography
GB/T 19681-2005 The method for the determination of Sudan dyes in foods-High performance liquid chromatography
GB/T 21317-2007 Determination of tetracyclines residues in food of animal origin—LC-MS/MS method and HPLC method
GB/T 26625-2011 Inspection of grain and oils—Determination of soybean isoflavone—High performance liquid chromatography
GB/T 26792-2019 High performance liquid chromatography
GB/T 26792-2011 High performance liquid chromatography
GB/T 40643-2021 Determination of hyperoside in extract of crataegus pinnatifida leaf—HPLC
GB/T 17592.2-1998 Textiles--Test method of the use of banned azo colourants--High pressure liquid chromatography method
GB/T 29636-2013 GC ,HPLC and GC-MS examination methods for methylamphetamine in suspected drug
BJS 202009 Determination of resorcinol in wheat flour by high performance liquid chromatography
GB/T 34782-2017 Method for the determination of poplar tree gum in propolis-High performance liquid chromatography
GB/T 38169-2019 Determination of caulerpin content—High performance liquid chromatography
GB/T 38479-2021 Determination of chitosan content―High performance liquid chromatography
GB/T 17078-1997 Workplace air--Determination of trinitrophenol--Liquid chromatographic method
GB/T 17528-2009 Determination of piperine content—Method using HPLC

Agricultural Standard of the People's Republic of China

农业部1025号公告-9-2008 Determination of doramectin residue in animal derived food High performance liquid chromatographic method
农业部1163号公告-6-2009 High performance liquid chromatographic method for the determination of Tylosin residues in edible tissues of swine and eggs
农业部1163号公告-5-2009 High performance liquid chromatographic method for determination of Ampkillin residues in edibh tissues
农业部1163号公告-7-2009 High performance liquid chromatographic method for determination of Gentamycin residues in animal food
农业部1025号公告-13-2008 Determination of Ceftiofur residue in animal tissues by high performance liquid chromatography
农业部1025号公告-10-2008 Determination of tilmieosin residue in animal derived food High performance liquid chromatographic method
农业部1862号公告-2-2012 Determination of zolpidem in feeds.High performance liquid chromatography/Liquid chromatography-tandem mass spectrometry
农业部1063号公告-4-2008 Determination of nadolol in feeds High-performance liquid chromatography
农业部1025号公告-15-2008 Determination of Sulfaquinoxaline residue in egg by high performance liquid chromatography

Jiangsu Provincial Standard of the People's Republic of China

DBS32/ 012-2016 High-performance liquid chromatography, liquid chromatography-mass spectrometry/mass spectrometry for the detection of quinoline yellow in local food safety standards

Professional Standard - Energy

NB/SH/T 0806-2022 Determination of aromatic hydrocarbon types in middle distillates-High performance liquid chromatography method with refractive index detector

Professional Standard - Petrochemical Industry

SH/T 0806-2008 Determination of aromatic hydrocarbon types in middle distillates-High performance liquid chromatography method with refractive index detection

Group Standards of the People's Republic of China

T/CSATC 5-2020 Determination of Silk? by High Performance Liquid Chromatography
T/CASME 713-2023 Determination of L-Carnosine—High performance liquid chromatography
T/CIRA 63-2024 Requirement for the use of radioactive flow detector in high performance liquid chromatography
T/WHAS 066-2024 Determination of gestrinone - High-performance liquid chromatography
T/NXFSA 004S-2020 Determination of Lycium barbarum by High Performance Liquid Chromatography
T/SDAS 619-2023 Determination of Anthocyanins in Color Corn by High Performance Liquid Chromatography

Professional Standard - Commodity Inspection

SN/T 2331-2016 Method for the ditermination of sugar in cotton fiber for import and export一 Hight performance liquid chromatography(HPLC)method
SN/T 2331-2009 Raw material of textiles—Determination of sugar contents in cotton—High performance liquid chromatography(HPLC)method
SN/T 3848-2014 Test method for tea polyphenol in food for export - High performance liquid chromatography
SN/T 4890-2017 Determination of curcuminoids in foodstuffs for export—HPLC method and HPLC-MS/MS method
SN/T 2544-2010 Determination of content in glycerine—High performance liquid chromatography
SN/T 5146-2019 Determination of L-carnitine in exported foods by high-performance liquid chromatography and liquid chromatography-mass spectrometry/mass spectrometry
SN/T 4799-2017 Detection of tuberculosis pathogenic organisms of animals. DHPLC method
SN/T 1974-2007 Determination of methylene bule residues in aquatic product for import and export LC-MS/MS and HPLC method
SN/T 2534-2010 Determination of Patulin in Fruit and Vegetable Products for Import and Export - LC-MS/MS and HPLC Method
SN/T 0528-2012 Determination of diflubenzuron residue in food for export. HPLC-MS/MS method
SN/T 4113-2015 Determination of acetophenon in plastic and plastic products-High performance liquid chromatography
SN/T 2152-2008 Determination of hexaflumuron residue in food for import and export—HPLC-MS/MS method

Professional Standard - Education

JY/T 024-1996 General rules for high performance liquid chromatography

Military Standard of the People's Republic of China-General Armament Department

GJB 3647A-2021 Determination of thiodiglycol by high performance liquid chromatography
GJB 4780A-2021 Determination of Sies by high performance liquid chromatography
GJB 4780-1997*GJBz 20413.2-1997 Determination of Sies by high performance liquid chromatography
GJB 4782-1997*GJBz 20414-1997 Determination of Benzene Chloroacetophenone by High Performance Liquid Chromatography
GJB 4782A-2020 Determination of Benzene Chloroacetophenone by High Performance Liquid Chromatography
GJB 3647-1999 Determination of thiodiglycol by high performance liquid chromatography

Professional Standard - Hygiene

WS/T 15-1996 Workplace air. Determination of benz (a)pyrene. High performance liquid chromatographic method
WS/T 98-1996 Urine - Determination of creatinine - Reversed - Phase high performance liquid chromatographic method

Professional Standard - Environmental Protection

HJ 1017-2019 Water quality—Determination of benzidine—High performance liquid chromatography
HJ 1071-2019 Water quality—Determination of glyphosate —High performance liquid chromatography
HJ 1073-2019 Water quality—Determination of naphthol —High performance liquid chromatography

Professional Standard - Grain

LS/T 6151-2024 Inspection of grains and oils-Determination of amygdalin content-High performance liquid chromatography

Shandong Provincial Standard of the People's Republic of China

DB37/T 3755-2019 Determination of 2,4-D in Fertilizer by High Performance Liquid Chromatography

Anhui Provincial Standard of the People's Republic of China

DB34/T 4207-2022 Determination of Ofloxacin in Chicken Excreta by High Performance Liquid Chromatography-Fluorescence Detection
DB34/T 2992-2017 Determination of optical brighteners in detergent products by high performance liquid chromatography

Professional Standard - Light Industry

QB/T 5715-2022 Determination of itaconic acid in fermentation liquor-High performance liquid chromatography method

Shanghai Provincial Standard of the People's Republic of China

DB31/T 822-2014 Determination of gossypol in feeds—HPLC method

Liaoning Provincial Standard of the People's Republic of China

DB21/T 3287-2020 Water Quality Determination of Acrolein by High Performance Liquid Chromatography

Standards Norway

NS-EN 15652:2009 Foodstuffs — Determination of niacin by HPLC
NS-EN 14164:2008 Foodstuffs — Determination of vitamin B6 by HPLC
NS-EN 14148:2003 Foodstuffs — Determination of vitamin K1 by HPLC
NS-EN 12916:2006 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
NS-EN 12857:1999 Foodstuffs — Determination of cyclamate — High performance liquid chromatographic method
NS-EN 14122:2014 Foodstuffs — Determination of vitamin B1 by high performance liquid chromatography
NS-EN 12916:2016 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
NS-EN 12916:2019 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
NS-EN 14152:2014 Foodstuffs — Determination of vitamin B2 by high performance liquid chromatography

Danish Standards Foundation

DANSK DS/EN 15652:2009 Foodstuffs - Determination of niacin by HPLC
DS/EN 15652:2009 Foodstuffs - Determination of niacin by HPLC
DANSK DS/EN 12916+A1:2022 Petroleum products – Determination of aromatic hydrocarbon types in middle distillates – High performance liquid chromatography method with refractive index detection
DS/EN 16162:2012 Animal feeding stuffs - Determination of decoquinate by HPLC with fluorescence detection
DS/EN 12916:2006 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
DS/EN 14152/AC:2006 Foodstuffs - Determination of vitamin B2 by HPLC
DANSK DS/EN 14152:2014 Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography
DANSK DS/EN 12857:1999 Foodstuffs - Determination of cyclamate - High performance liquid chromatographic method
DANSK DS/EN 12916:2019 Petroleum products – Determination of aromatic hydrocarbon types in middle distillates – High performance liquid chromatography method with refractive index detection
DS/EN 15607:2009 Foodstuffs - Determination of d-biotin by HPLC
DS/EN 14122/AC:2006 Foodstuffs - Determination of vitamin B1 by HPLC
DS/EN 14164:2008 Foodstuffs - Determination of vitamin B6 by HPLC
DANSK DS/EN 14152/AC:2006 Foodstuffs - Determination of vitamin B2 by HPLC
DS/EN 12857:1999 Foodstuffs - Determination of cyclamate - High performance liquid chromatographic method
DANSK DS/EN 14122:2014 Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography

Ente Nazionale Italiano di Unificazione

UNI EN 15652:2009 Foodstuffs - Determination of niacin by HPLC
UNI EN 16162:2012 Animal feeding stuffs - Determination of decoquinate by HPLC with fluorescence detection
UNI EN 14152:2014 Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography
UNI EN 12857:1999 Foodstuffs - Determination of cyclamate - High performance liquid chromatographic method

Asociación Española de Normalización (AENOR)

AENOR UNE-EN 16162:2012 Animal feeding stuffs - Determination of decoquinate by HPLC with fluorescence detection
AENOR UNE-EN 15652:2010 High-performance liquid chromatography determination of niacin in food
AENOR UNE-EN 14122:2014 Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography
AENOR UNE-EN 14148:2004 FOODSTUFFS. DETERMINATION OF VITAMIN K1 BY HPLC.

German Institute for Standardization

DIN EN 15652:2009-09 Foodstuffs - Determination of niacin by HPLC; German version EN 15652:2009
DIN EN 12916/A1:2021 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection; German and English version EN 12916:2019/prA1:2021
DIN EN 12916:2019 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
DIN EN 15652 E:2007-05 Draft Document - Foodstuffs - Determination of niacin by HPLC; German version prEN 15652:2007
DIN EN 14122:2014-08 Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography; German version EN 14122:2014
DIN EN 12857:1999-07 Foodstuffs - Determination of cyclamate - Method by high performance liquid chromatography; German version EN 12857:1999
DIN EN 12916:2023-07 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection; German and English version prEN 12916:2023 / Note: Date of issue 2023-06-02*Intended...
DIN EN 14148:2003-10 Foodstuffs - Determination of vitamin K1 by HPLC; German version EN 14148:2003
DIN EN 14152:2003 Foodstuffs - Determination of vitamin B2 by HPLC; German version EN 14152:2003
DIN EN 12916:2023 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection; German and English version prEN 12916:2023
DIN EN 14152:2014-08 Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography; German version EN 14152:2014
DIN EN 14148:2003 Foodstuffs - Determination of vitamin K1 by HPLC; German version EN 14148:2003

Lithuanian Standards Office

LST EN 15652-2009 Foodstuffs - Determination of niacin by HPLC
LST EN 16162-2012 Animal feeding stuffs - Determination of decoquinate by HPLC with fluorescence detection
LST EN 12916-2006 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
LST EN 14152-2004 Foodstuffs - Determination of vitamin B2 by HPLC
LST EN 14152-2004/AC-2005 Foodstuffs - Determination of vitamin B2 by HPLC
LST EN 14122-2004 Foodstuffs - Determination of vitamin B1 by HPLC
LST EN 15607-2009 Foodstuffs - Determination of d-biotin by HPLC
LST EN 12857-2000 Foodstuffs - Determination of cyclamate - High performance liquid chromatographic method

Spanish Association for Standardization (UNE)

UNE-EN 15652:2010 Foodstuffs - Determination of niacin by HPLC
UNE-EN 16162:2012 Animal feeding stuffs - Determination of decoquinate by HPLC with fluorescence detection
UNE-EN 15607:2010 Foodstuffs - Determination of d-biotin by HPLC
UNE-EN 14152:2015 Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography
UNE-EN 14164:2008 Foodstuffs - Determination of vitamin B6 by HPLC
UNE-EN 12857:2001 Foodstuffs - Determination of cyclamate - High performance liquid chromatographic method
UNE-EN 12916:2007 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
UNE-ENV 14164:2007 EX Foodstuffs - Determination of vitamin B6 by HPLC
UNE-EN 12916:2019+A1:2023 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
UNE-EN 14122:2014 Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography

Korean Agency for Technology and Standards (KATS)

KS M 2456-2014(2024) Petroleum product — Determination of aromatic hydrocarbon types in middle distillates — High performance liquid chromatography method with refractive index detection
KS I ISO 11264-2016 Soil quality－Determination of herbicides－Method using HPLC with UV-detection
KS M 2456-2014 Petroleum product — Determination of aromatic hydrocarbon types in middle distillates — High performance liquid chromatography method with refrN index detection
KS I ISO 11264-2016(2021) Soil quality－Determination of herbicides－Method using HPLC with UV-detection
KS I ISO 11264-2021 Soil quality－Determination of herbicides－Method using HPLC with UV-detection
KS M 0033-2008(2023) General rules for analytical methods in high performance liquid chromatography
KS M 0033-2023 General rules for analytical methods in high performance liquid chromatography
KS M 0033-1993 General rules for analytical methods in high performance liquid chromatography
KS M 2456-2024 Petroleum product — Determination of aromatic hydrocarbon types in middle distillates — High performance liquid chromatography method with refractive index detection

Swedish Institute for Standards

SS-EN 15705:2023 Inorganic fertilizers - Determination of methylen-urea oligomers using high-performance liquid chromatography (HPLC)
SS-EN 12916:2019+A1:2022 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
SS-EN 17550:2021 Animal feeding stuffs: Methods of sampling and analysis - Determination of carotenoids in animal compound feed and premixtures by high performance liquid chromatography - UV detection (HPLC-UV)
SS-EN 12857:1999 Foodstuffs - Determination of cyclamate - High performance liquid chromatographic method
SS-EN 16155:2012 Foodstuffs - Determination of sucralose - High performance liquid chromatographic method

Japanese Industrial Standards Committee (JISC)

JIS K 0124:2002 General rules for high performance liquid chromatography
JIS K 0124:2011 General rules for high performance liquid chromatography
JIS K 0124:1994 General rules for high performance liquid chromatographic analysis

British Standards Institution (BSI)

BS ISO 16308:2014 Water quality. Determination of glyphosate and AMPA. Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection
BS EN 15905:2010 Fertilizers - Determination of 3-methylpyrazole (MP) using high-performance liquid chromatography (HPLC)
BS EN 15652:2009 Foodstuffs - Determination of niacin by HPLC
BS EN 15607:2009 Foodstuffs - Determination of d-biotin by HPLC

International Organization for Standardization (ISO)

ISO 16308:2014 Water quality - Determination of glyphosate and AMPA - Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection

Association Francaise de Normalisation

NF T90-189*NF ISO 16308:2014 Water quality - Determination of glyphosate and AMPA - Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection
NF ISO 11264:2008 Soil quality - Herbicide dosage - HPLC method with UV detection
NF EN 15652:2009 Food products - Determination of niacin by High Performance Liquid Chromatography (HPLC)
NF M07-074*NF EN 12916:2019 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
NF V03-161*NF EN 15652:2009 Foodstuffs - Determination of niacin by HPLC
NF EN 12916/IN1:2022 Petroleum products - Determination of aromatic hydrocarbon families in middle distillates - High performance liquid chromatography method with differential refractometry detection
NF EN 12916+A1:2022 Petroleum products - Determination of aromatic hydrocarbon families in middle distillates - High performance liquid chromatography method with differential refractometry detection
NF V03-134:2004 Foodstuffs - Determination of vitamin B2 by HPLC.
NF EN 12857:2001 Food products - Determination of cyclamate - High performance liquid chromatography method
NF V03-160*NF EN 15607:2009 Foodstuffs - Determination of d-biotin by HPLC.
NF V03-134*NF EN 14152:2014 Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography

European Committee for Standardization (CEN)

EN 12916:2016 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
EN 12916:2024 Petroleum products - Determination of aromatic hydrocarbon types in middle distillates - High performance liquid chromatography method with refractive index detection
PREN 14152-2012 Foodstuffs - Determination of vitamin B2 by HPLC

Kingdom of Bahrain Testing and Metrology Directorate

BH GSO ISO 16308:2017 Water quality -- Determination of glyphosate and AMPA -- Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection
BH GSO ISO 11264:2016 Soil quality -- Determination of herbicides -- Method using HPLC with UV-detection

GCC Standardization Organization

GSO ISO 16308:2015 Water quality -- Determination of glyphosate and AMPA -- Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection
GSO ISO 13859:2015 Soil quality -- Determination of polycyclic aromatic hydrocarbons (PAH) by gas chromatography (GC) and high performance liquid chromatography (HPLC)

The Directorate General of Specifications and Metrology (DGSM)

OS GSO ISO 11264:2014 Soil quality -- Determination of herbicides -- Method using HPLC with UV-detection

Gosstandart of Russia

GOST 34151-2017 Foodstuffs. Determination of vitamin C by high performance liquid chromatography
GOST EN 15652-2015 Foodstuffs. Determination of niacin by high performance liquid chromatography
GOST R EN 12857-2010 Foodstuffs. Determination of cyclamate. High performance liquid chromatographic method
GOST EN 12857-2015 Foodstuffs. Determination of cyclamate. High performance liquid chromatographic method
GOST EN 15360-2015 Fertilizers. Determination of dicyandiamide by high-performance liquid chromatography method