SN/T 4890-2017 in English
VALIDDetermination of curcuminoids in foodstuffs for export—HPLC method and HPLC-MS/MS method
- Issued on:2017-08-29
- Implemented on:2018-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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Introduction
Analysis of the Standard's Core Content
This standard specifies the detection method for curcuminoids (Cur I, Cur II, and Cur III) in food for export. It uses two technical approaches: high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS). The limits of determination are 10 mg/kg (HPLC) and 1.0 mg/kg (LC-MS/MS), respectively, covering 13 food matrices.
Comparative analysis of methods
| Comparison dimensions | HPLC method | LC-MS/MS method |
|---|---|---|
| Detection principle | UV detector (420nm) | Electrospray ionization source (ESI+) |
| Chromatographic column | C18 column (4.6×250mm,5μm) | C18 column (2.1×150mm,3μm) |
| Analysis time | 18 min/sample | 12 minutes/sample |
| Sensitivity | 10mg/kg | 1.0mg/kg |
Key Technical Points
Sample Pretreatment Differences
For high-fat samples (such as ice cream and milk powder), a polyamide solid-phase extraction cartridge is required for cleanup. The eluent is 5% methanol in water and the elution is 2% ammoniated methanol. Due to the higher sensitivity of the LC-MS/MS method, the sample volume can be reduced to 2.5mL.
Mass spectrometry parameter optimization
LC-MS/MS was performed in multiple reaction monitoring (MRM) mode, and the quantitative ion pair for CurⅠ was 369.0→177.2 (CE=45eV). It should be noted that the DP voltage (51V) and collision energy needed to be re-optimized for different instruments.
Implementation Recommendations
- HPLC is the preferred method for routine screening, as it is low-cost and meets most testing needs.
- For samples with low limit requirements, such as infant food, LC-MS/MS must be used.
- Note that the reference materials should be stored in the dark (0-4°C). The CAS numbers of the three curcumin standards are shown in Appendix A.
- For complex matrices, it is recommended to add matrix-matched standard curves to reduce ion suppression.
Background of Standard Evolution
This standard was published in 2017. Compared to the earlier GB/T 22244-2008, the main improvements include: ① the addition of LC-MS/MS confirmation methods; ② expansion to 13 food matrices; and ③ optimization of the solid-phase extraction process. Even after the 2020 amendment to Regulation (EU) No 1129/2011 by the European Union, this standard remains technologically advanced.
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