GB/T 38479-2021 in English

Technical background and evolution of standards

As the first national standard in my country specifically for the determination of chitosan content, this standard fills the gap in the standardization of quantitative analysis of chitosan derivatives. Compared with traditional chemical titration, high performance liquid chromatography (HPLC) has significant advantages such as high sensitivity (detection limit 0.30mg/mL) and good repeatability (RSD≤1.5%).

Core method principle

Chitosan is converted into chitosan through acetylation reaction, and then hydrolyzed with gradient acid to generate glucosamine hydrochloride, which is separated by amino column (250mm×4.6mm, 5μm), detected by evaporative light scattering detector (ELSD), and quantified by external standard method. Key technological breakthroughs include:

Key Operating Points

Implementation suggestions

1. The laboratory needs to be equipped with special equipment such as constant temperature magnetic stirrer and rotary evaporator
2. The standard curve needs to cover the range of 1.0-3.5mg/mL, and the correlation coefficient R²≥0.999
3. Each batch of determination should include blank control and quality control sample

Expansion of application areas

This method is not only applicable to the determination of pure chitosan, but can also be used for the following after method validation:

• Quality monitoring of chitin products
• Detection of chitosan content in medical dressings
• Quantitative analysis of functional food additives