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Database: 365,228(8 Aug 2026)
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GB/T 41907-2022 in English

GB/T 41907-2022 in English

VALID

Assay method of enterokinase activity

  • Issued on:2022-10-12
  • Implemented on:2022-10-12
  • File Format:PDF
  • Delivery:Via email within 1~3 business days
Price(USD): $120.00
$117.00
Standard No: GB/T 41907-2022
Document status: VALID
Title in English: Assay method of enterokinase activity
Title in Chinese: 肠激酶活性检测方法
Language: English
File Format: Electronic (PDF)
Delivery: Via email within 1~3 business days
Issued on: 2022-10-12
Implemented on: 2022-10-12
ICS Classification: 07.080-Biology. Botany. Zoology
Chinese Classification: C27-Biological agent and blood products
Professional Classification: GB-National Standard
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GB/T 41907
GB/T 41907-2022
Method for Determining Enzyme Activity
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《GB/T 41907-2022肠激酶活性检测方法》由SWG11(全国工具酶标准化工作组)归口,主管部门为国家标准化管理委员会。


Introduction

Professional Standard Technical Analysis

This document specifies an accurate detection scheme for the thioredoxin-NP27 enzyme cleavage reaction with the support of a electrophoresis instrument. The core is to establish a standardized buffer system (pH range 8.4±0.2) and strictly limit the 16 hours±0.5℃ enzymatic reaction conditions.

Comparative Analysis of Technical Solutions

Technical Elements Current Standards Historical Methods
Temperature Control Accuracy ±0.5℃ ±1℃
Protein Concentration Detection Standard Steps for UV Spectrophotometry ELISA Estimation
Quality Management Requirements Repeatability Difference <10% No Quantitative Regulations

Demonstration Cases of Key Steps

Verification with standard case: After adding

Refer to GB/T6682 first-level water standard, pay special attention to phenol blue color development delay> 2 minutes, which indicates that the electrophoresis solution ion concentration is abnormal, and the reagent Tween20 content needs to be re-calibrated to see if it meets the 0.1% requirement.

Recommendations for the Implementation of the Standard

  • New laboratories should establish a chromatographic column calibration control record system
  • It is recommended to use the

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