GB/T 41907-2022 in English
VALIDAssay method of enterokinase activity
- Issued on:2022-10-12
- Implemented on:2022-10-12
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 41907-2022肠激酶活性检测方法》由SWG11(全国工具酶标准化工作组)归口,主管部门为国家标准化管理委员会。
Introduction
Professional Standard Technical Analysis
This document specifies an accurate detection scheme for the thioredoxin-NP27 enzyme cleavage reaction with the support of a
Comparative Analysis of Technical Solutions
| Technical Elements | Current Standards | Historical Methods |
|---|---|---|
| Temperature Control Accuracy | ±0.5℃ | ±1℃ |
| Protein Concentration Detection | Standard Steps for UV Spectrophotometry | ELISA Estimation |
| Quality Management Requirements | Repeatability Difference <10% | No Quantitative Regulations |
Demonstration Cases of Key Steps
Verification with standard case: After adding Refer to GB/T6682 first-level water standard, pay special attention to phenol blue color development delay> 2 minutes, which indicates that the electrophoresis solution ion concentration is abnormal, and the reagent Tween20 content needs to be re-calibrated to see if it meets the 0.1% requirement.
Recommendations for the Implementation of the Standard
- New laboratories should establish a chromatographic column calibration control record system
- It is recommended to use the
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