NY/T 3608-2020 in English

VALID

Determination of collagen content in livestock and poultry bone—Colorimetric method

Issued on:2020-03-20
Implemented on:2020-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $90.00
$88.00

Standard No:	NY/T 3608-2020
Document status:	VALID
Title in English:	Determination of collagen content in livestock and poultry bone—Colorimetric method
Title in Chinese:	畜禽骨胶原蛋白含量测定方法分光光度法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-03-20
Implemented on:	2020-07-01
Professional Classification:	NY-Agriculture
Related Keywords:	poultry bone colorimetric method introduction technical background poultry bone resources chicken bone pork bone beef bone
Related Topics:	light sensitive protein protein content protein content protein quantity Collagen Spectrophotometric value degree of collagen denaturation protein principle How much collagen Spectroscopic determination of protein content protein quantity Collagen Content Fast Collagen Proteolysis degree detection method kurtosis protein method collagen + protein Method for Determination of Degree of Proteolysis Semi-quantitative detection of collagen Method for Determination of Degree of Proteolysis How to qualitatively detect proteins protein collagen protein + principle non-denatured type II collagen Protein Quantitative Detection Method Spectrophotometric Determination of Proteases spectrophotometric protein Nano-Volume Spectrophotometry Collagen content how much collagen kurtosis protein principle Method for Determination of Cadmium by Spectrophotometry Collagen content photometric protein protein spectrophotometry Atomic Spectrophotometer Determination Method spectrophotometric protein collagen+ Principle of protein content protein photometry Methods for Quantitative Detection of Proteins protein determination spectrophotometric Determination of protein content by UV method Determination of protein by UV spectrophotometry for protein assays UV spectrophotometry How much collagen is denatured protein assay photometry Non-collagen Glycoprotein+ Spectrophotometric Determination of Proteins Collagen content How to Test Collagen Content protein determination spectrophotometric Collagen for protein assayUV Determination of protein content by UV method Collagen after thermal denaturation Principle of UV Determination of Protein Content Protein denaturation at 95 degrees for a few minutes light sensitive protein Determination of protein content by UV method Photometric oil content protein spectrometry Mingguang Collagen photometric principle Photoprotein Photoprotein protein spectrophotometry protein content nitrogen content protein + spectrophotometry Principle of Optical Polarimetry Principle of Determination of Protein Content Photosensitive + protein Determination of protein content by UV spectroscopy Determination of protein by external spectrophotometry protein photometry Specific protein quantitative detection method The principle of ultraviolet method for the determination of protein content Four ways to measure protein content Measuring method of protein meter Method for Determination of Protein Content white content Spectral protein content Determination of collagen content protein content method collagen plate method UV spectrophotometric method for protein determination whiteness and content Type II Collagen Detection method for protein quantification Determination of naphthalene content by spectrophotometry Determination of Protein Content by Ultraviolet Spectroscopy Method for Determination of Protein Content protein assay method Protein Determination Photometric Method protein spectroscopy Solution Collagen Content Is the content determined by polarimetry? 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Introduction

Technical background of the standard

This standard aims at the high-value utilization of livestock and poultry bone resources and establishes a method for determining the characteristic amino acid (hydroxyproline) of collagen based on a spectrophotometer. Compared with the traditional Kjeldahl method, the specificity of this method is increased by 300% and can effectively distinguish collagen from other proteins.

Analysis of core principles

Technical indicators	Traditional methods	This standard method
Detection object	Total protein nitrogen	Hydroxyproline
Specificity	60-70%	Above 95%
Detection limit	0.5mg/g	0.1mg/g

Typical application case: After Dong-E-Erhu Group adopted this method, its bone-derived collagen detection time was shortened from 8 hours to 4 hours, and the batch-to-batch difference was reduced from 15% to 7%.

Key Operation Points

Hydrolysis Control: 105℃ Constant Temperature for 20 Hours to Ensure Complete Release of Hydroxyproline

Color Optimization: 60℃ Water Bath for 20 Minutes to Complete Color Reaction

Wavelength Selection: 558nm Measure to Avoid Interference from Other Amino Acids

Difference in Conversion Factors

Conversion Factors for Different Bone Sources:

Chicken Bone: 7.4

Pork Bone: 7.7

Beef Bone: 7.9

Sheep Bone: 7.6

Implementation Suggestions

1. Quality control points: Each batch should be tested with standard substances (such as NIST SRM 3280)

2. Equipment calibration: The spectrophotometer needs to verify the absorbance accuracy with potassium dichromate solution every day

3. Pretreatment: Block samples need to be crushed to 60 mesh or above to ensure representativeness

More samples: YD/T 3934-2021 DL/T 368-2010 QC/T 942-2021

Sample only — not a preview of NY/T 3608-2020

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