SN/T 5276-2020 in English

VALID

Determination of multiple-allergens in foodstuff of export - LC-MS/MS method

Issued on:2020-08-27
Implemented on:2021-03-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $250.00
$243.00

Standard No:	SN/T 5276-2020
Document status:	VALID
Title in English:	Determination of multiple-allergens in foodstuff of export - LC-MS/MS method
Title in Chinese:	出口食品中多种过敏原的测定液相色谱-质谱/质谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-08-27
Implemented on:	2021-03-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	lc-ms/ms method introduction analysis traditional elisa method 65.5-93.6 % method innovation hydrophobic protein method
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Introduction

Analysis of standard technical framework

Detection object	Number of characteristic peptides	Limit of quantification (mg/kg)	Recovery rate range
Milk protein	4	5	66.3-98.9%
Egg protein	1	5	67.1-92.3%
Nut protein	5	10	65.5-93.6%

Method innovation

The recovery rate of hydrophobic protein was significantly improved by combining Tris-HCl buffer with 60℃ heat extraction technology. The matrix interference was effectively reduced by 10kDa ultrafiltration and trypsin-directed enzymatic hydrolysis strategies.

Key operating points

Samples with fat content ＞5% need to be defatted with n-hexane
Alkylation reaction must be strictly protected from light
Mass spectrometry acquisition adopts dynamic MRM mode

Implementation suggestions

Biscuit sample testing case: Weigh 2.0g of crushed sample, defatted it twice with n-hexane, and extracted according to clause 4.2.9. The final peanut allergen content was detected to be 28.6mg/kg, with RSD=5.3%.

Technology Evolution Analysis

Compared with the traditional ELISA method, this standard:

Detection throughput increased by 3 times
Can detect 7 types of allergens simultaneously
Specificity increased to 100%

More samples: JC/T 2453-2018 JGJ 94-2008 GB 18306-2015

Sample only — not a preview of SN/T 5276-2020

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