GB/T 5009.82-2003 in English

SUPERSEDED

Determination of retinol and tocopherol in foods

Issued on:2003-08-11
Implemented on:2004-01-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $60.00
$59.00

Standard No:	GB/T 5009.82-2003
Document status:	SUPERSEDED
Superseded by:	GB 5009.82-2016 National Food Safety Standard -- Determination of Vitamins A, D and E in Foods
Superseded on:	2017-12-30
Title in English:	Determination of retinol and tocopherol in foods
Title in Chinese:	食品中维生素A和维生素E的测定
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2003-08-11
Implemented on:	2004-01-01
Superseding:	GB/T 12388-1990 Method for determination of retinol and tocopherol in foods
ICS Classification:	67.040-food products in general
Chinese Classification:	C53-Food sanitation
Professional Classification:	GB-National Standard
Related Keywords:	scope method precision method internal standard method high performance liquid chromatography high performance liquid chromatography c18
Related Topics:	Vitamin E vitamins Vitamin A Vitamin B Vitamin D Vitamin B12 food grade vitamin c standard food grade vitamin c food grade vitamin c vitamin e reagent Vitamin A Three Points vitamin c food grade white vitamin b3 in food Mass Spectrometry Vitamin Detection vitamin c food grade food grade vitamins vitamin spectrum vitamin a+ Vitamin detection by mass spectrometry vitamin d in oil vitamin e vitamin a Vitamin mass spectrometry detection Vitamin mass spectrometry detection Vitamin 14 items vitamin d foods vitamin content vitamins in serum Vitamin A UV vitamin c content in vitamin c tablets Detecting Antibiotics in Food vitamin c food vitamin b4 tablets vitamin b4 vitamin b4 quality food vitamin b3 Vitamin liquid detection vitamins in food vitamin content in fruit Vitamin this period vitamin c vitamin gb5009.82 brand vitamins Determination of vitamin b3 in food vitamin brand vitamin food vitamin a+ peak UV vitamin A content Vitamin + Peak vitamin c test Determination of Vitamin C Content in Food Check vitamin content Determination of food coloring Vitamin content detection food vitamin c measure vitamin c Vitamin content in food UV test vitamin B3 Vitamin b5 and provitamin b5 in cosmetics vitamin effect vitamin c in food vitamins in fruit vitamin b7 Vitamins and Multivitamins Prime+ Determination of Vitamin C in Food for vitamin E content determinationUV Food Vitamin C Determination for vitamin content determinationUV Vitamin Analysis Vitamin detection mass spectrometry Vitamin A UV Provitamin B5 vs Vitamin B5 Determination of Vitamin C by Titration fiber food Vitamin detection mass spectrometry Provitamin B5 Vitamin B5 Vitamin Analysis vitamins in fruit vitamin d foods vitamin d food Detection of vitamin b3 Determination of Vitamins in Fruits vitamin e content uv broad spectrum vitamin e vitamin sec Broad Spectrum Vitamins parameters of vitamin c Spectrum Vitamins vitamin content Nine kinds of vitamin detection How to test vitamin b3 Five vitamin mass spectrometry detection vitamin b4 content vitamin raw material three elements of food Lithium in food food grade vitamin e quality How to measure vitamins in food Rapid analysis of vitamins in food Detection of Antibiotics in Water vitamin use Determination of Vitamin A Content by Ultraviolet Method vitamin b5 or provitamin b vitamin a1 vitamin k2 and vitamin c vitamin group testing Vitamin b3 detection to survive Kilocytoma Vein tumor vitamin form Vitamin A+ UV Broad Spectrum Vitamins vitamin c foods vitamin impurities fourteen vitamins Magnesium in food Vitamin C past and present Vitamin+ Spectrum Vitamins food vitamin vitamin meter vitamin b5 and provitamin b5 vitaminization method daily vitamin e maximum Vitamin E UV Determination of Vitamin C by Ultraviolet Method vitamin a in food vitamin c content in food How is Vitamin C Measured? vitamin c foods vitamin d foods elements of organic food 4 vitamins 5 vitamins vitamin a in food vitamin e vitamin food vitamin c content vitamin long term k vitamin foods antibiotic food vitamin food vitamin c food vitamin k food Chromatographic Analysis of Vitamins vitamin extraction Vitamin D Analysis food vegetarian vitamin c content in vitamin c Specific Absorption Coefficient vitamin d threshold Identification of Vitamin C vitamin selenium Vitamin A content in feed food vitamin Determination of two levels of vitamin A vitamin test method vitamin extract Retinol UV vitamin b2 and vitamin c ten Vitamin E Analysis Vitamin B content GB/T 5009.82 GB/T 5009.82-2003 Determination method of vitamin A in food in food Physical examination vitamin A 885 Colorimetric determination of vitamin E Chemical instruments and methods for measuring vitamin A Three-point calibration method for determination of vitamin A content Agilent Vitamins Disadvantages of Fluorescence Method for Determining Vitamin C Determination of Vitamin C by Potentiometric Titration Three-point calibration method for determination of vitamin A Water antibiotic determination How to measure vitamins in fruits Testing methods for vitamins in soft capsules Foreign methods for measuring vitamins Detection methods for antibiotics in food cellulase food Antibiotics determination in food Methods for determining vitamins Method for labeling avidin-biotin

《GB/T 5009.82-2003食品中维生素A和维生素E的测定》由361（国家卫生健康委员会）归口，主管部门为国家卫生健康委员会。
本标准规定了食品中维生素A和维生素E的测定方法。本标准适用于食品中维生素A和维生素E的测定。

1 Scope

This standard specifies the method for the determination of retinol and tocopherol in foods.
This standard is applicable to the determination of retinol and tocopherol in foods.
The detection limits of this standard are: VA: 0.8ng; α-E: 91.8ng; γ-E: 36.6ng; δ-E: 20.6ng.

Method I High Performance Liquid Chromatography

2 Principle

After saponification treatment, retinol and tocopherol in the specimen shall be extracted from the non-saponifiable part into the organic solvent, separated with high performance liquid chromatography C18 reversed phase column, detected by UV detector and then determined quantitatively with internal standard method.

Contents
Foreword I
1 Scope
Method I High Performance Liquid Chromatography
2 Principle
3 Reagents
4 Instruments and Equipment
5 Analysis Procedures
6 Result Calculation
7 Precision
Method 2: Colorimetric Method
8 Principle
9 Reagents
10 Instruments
11 Analysis Procedures
12 Determination
13 Result calculation
14 Precision

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