GB/T 34793-2017 in English
VALIDProteinase K
- Issued on:2017-11-01
- Implemented on:2018-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 34793-2017蛋白酶K》由424-cnis(中国标准化研究院)归口,主管部门为国家市场监督管理总局。
Introduction
In-depth interpretation of the National Standard of the People's Republic of China GB/T 34793—2017
| Comparative analysis of standard framework | ||
|---|---|---|
| Dimensions | Technical requirements | Test methods |
| Sensory requirements | Solid: powder, white; liquid: liquid, colorless and transparent | Visual inspection method, in line with GB/T 191 Packaging, storage and transportation graphic symbols |
| Physical and chemical indicators | Proteinase K enzyme activity, ribonuclease enzyme activity, deoxyribonuclease enzyme activity, etc. | In line with GB/T 34800, GB/T 34222, GB/T 34801 detection standards |
| Relative molecular mass determination | SDS-PAGE method, see Appendix A | Sodium dodecyl sulfate-polyacrylamide gel electrophoresis method |
Case analysis of proteinase K in practical application
Background introduction: Proteinase K is a serine protease mainly used to hydrolyze natural keratin. It is widely used in biotechnology, food industry and medicine. For example, in nucleic acid testing, proteinase K can be used to lyse cell membranes, releasing DNA and RNA.
Background of Standard Formulation
GB/T 34793—2017 was formulated based on the following needs:
- Unify the terms and definitions of proteinase K
- Standardize its technical requirements and detection methods
- Adapt to the development needs of the domestic biotechnology industry
Technology Evolution Analysis
From the historical version to GB/T 34793—2017, the proteinase K standard has undergone the following evolution:
- The definition of the enzyme activity unit is more precise (U represents)
- The detection method introduces more advanced SDS-PAGE technology
- Storage conditions are more stringent
Implementation Recommendations
- Manufacturers: It is recommended to conduct type inspections regularly to ensure that the products meet national standards.
- Testing agencies: Use the GB/T series methods specified in the standards for testing.
- Research institutions: When citing this standard in research, attention should be paid to the determination requirements of enzyme activity and relative molecular mass.

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